The shapefiles of the global biogeographical regions of freshwater fishes are available below:

• Regions and supercontinental regions: zip file
• Supercontinental regions only: zip file
• Regions only : zip file

For those interested in the methods we used in the paper, I wrote a complete, detailed tutorial on how to perform biogeographical network analyses in R here: https://github.com/Farewe/biogeonetworks